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Generate A Clonal Cell Line Protocol
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Originally published: 29/03/2018 16:40
Last version published: 30/03/2018 10:26
Publication number: ELQ-49846-2
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Generate A Clonal Cell Line Protocol

This technote details a 3-step protocol to generate a clonal cell line using Lentiviral Vectors.

GEG Techby GEG Tech
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cell biologygenetic engineeringgene transfercell culturesciencescientists
Description
For your research project purposes, you may need a clonal cell line which stably expresses your protein of interest over time. Lentiviral vectors allow you to create a clonal cell line over-expressing a transgene of interest. Such cell line can be developed following this 3-step protocol, validated on HEK293T cells and HeLa cells.

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